Comprehensive Course in Molecular Genetics
1- Introduction to genetic databases and primer design
2- Introduction to PCR and electrophoresis
3- Real-Time PCR
Headline
- Introduction to the principles of biosafety in the laboratory
- Introduction to and work with molecular laboratory equipment
- Extraction of nucleic acids (DNA and RNA)
- Methods for measuring the concentration and purity of nucleic acids
- Primer design (comprehensive introduction to genetic databases, principles, and primer design)
- Introduction to the principles and applications of PCR
- Practical implementation of PCR steps
- Introduction to different types of PCR (PCR-RFLP, ARMS PCR, Gap-PCR, Multiplex-PCR, Tetra ARMS PCR)
- Introduction to electrophoresis and detection of DNA fragments
- Analysis of the results of the DNA fragment pattern in electrophoresis
- Investigation of work errors and problem-solving (PCR Troubleshooting)
- cDNA synthesis
- Real-time PCR in research and research
- Real-time PCR in disease diagnosis
- Investigation of work errors and problem-solving (Real-Time PCR Troubleshooting)
- Sanger Sequencing analysis
Audience: Students and researchers in general Medicine, Genetics, Cellular-Molecular, Biotechnology and other Biological disciplines
Along with presenting a valid certificate from the Endocrinology and Metabolism Research Institute (EMRI)