Jan 29 2025
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Metabolic Disorders Research Center

 

 

 

Comprehensive Course in Molecular Genetics

1- Introduction to genetic databases and primer design

2- Introduction to PCR and electrophoresis

3- Real-Time PCR

 

Headline

  • Introduction to the principles of biosafety in the laboratory
  • Introduction to and work with molecular laboratory equipment
  • Extraction of nucleic acids (DNA and RNA)
  • Methods for measuring the concentration and purity of nucleic acids
  • Primer design (comprehensive introduction to genetic databases, principles, and primer design)
  • Introduction to the principles and applications of PCR
  • Practical implementation of PCR steps
  • Introduction to different types of PCR (PCR-RFLP, ARMS PCR, Gap-PCR, Multiplex-PCR, Tetra ARMS PCR)
  • Introduction to electrophoresis and detection of DNA fragments
  • Analysis of the results of the DNA fragment pattern in electrophoresis
  • Investigation of work errors and problem-solving (PCR Troubleshooting)
  • cDNA synthesis
  • Real-time PCR in research and research
  • Real-time PCR in disease diagnosis
  • Investigation of work errors and problem-solving (Real-Time PCR Troubleshooting)
  • Sanger Sequencing analysis

 

Audience: Students and researchers in general Medicine, Genetics, Cellular-Molecular, Biotechnology and other Biological disciplines

Along with presenting a valid certificate from the Endocrinology and Metabolism Research Institute (EMRI)

 

 

 

 

 

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