Headline

Workshop

Introduction to the principles of biosafety in the laboratory

Introduction to and work with molecular laboratory equipment

Extraction of nucleic acids (DNA and RNA)

Methods for measuring the concentration and purity of nucleic acids

Primer design (comprehensive introduction to genetic databases, principles, and primer design)

Introduction to the principles and applications of PCR

Practical implementation of PCR steps

Introduction to different types of PCR (PCR-RFLP, ARMS PCR, Gap-PCR, Multiplex-PCR, Tetra ARMS PCR)

Introduction to electrophoresis and detection of DNA fragments

Analysis of the results of the DNA fragment pattern in electrophoresis

Investigation of work errors and problem-solving (PCR Troubleshooting)

cDNA synthesis

Real-time PCR in research and research

Real-time PCR in disease diagnosis

Investigation of work errors and problem-solving (Real-Time PCR Troubleshooting)

Sanger Sequencing analysis

Introduction to Genetic Databases and Primer Design

Introduction to PCR and Electrophoresis

Real-Time PCR

Introduction to Next Generation Sequencing (NGS)

Linux Installation and Training

First-Step Analysis of NGS Data (Whole Exome Sequencing Data (WES))

Second-Step Analysis of NGS Data

Post-Alignment

Variant Calling

Third-Step Analysis of NGS Data

Interpretation of Results

Practice (Performing Steps for 2 Patients) and Troubleshooting

Use of genetic technology and its applications in diagnosis and clinical research: Analysis of NGS data (DNA Sequencing)

Audience: Students and researchers in general Medicine, Genetics, Cellular-Molecular, Biotechnology, Bioinformatics, and other Biological disciplines

Along with presenting a valid certificate from the Endocrinology and Metabolism Research Institute (EMRI)